Kalirin-7 plays the neuroprotective role in Neuro-2A cells injured by oxygen-glucose deprivation and reperfusion through Rac1 activation

Kalirin-7 plays the neuroprotective role in Neuro-2A cells injured by oxygen-glucose deprivation and reperfusion through Rac1 activation

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Objective(s): The study explored the neuroprotective role of Kalirin-7 (Kal-7) in Neuro-2A cells after oxygen-glucose deprivation and reperfusion (OGD/R) treatment.Materials and Methods: The study used an OGD/R model of mouse Neuro-2A neuroblastoma cells in vitro.Cells were transfected with pCAGGS-Kal-7 to up-regulating kal-7.Then cell proliferation Quarter Zip and apoptosis were respectively analyzed by Assembly Parts (OE Style) Trypan blue exclusion method and flow cytometry.

To examine the involvement of Rac1, cells were treated with Rac1-GTP inhibitor NSC23766 before treatment with OGD/R.Expressions of Bax, Bcl-2, Rac1, and down-stream targets of Rac1 were analyzed by Western blot.Results: Kal-7 significantly decreased OGD/R induced cell apoptosis (P.